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Ahn, Jee-Yin, Assistant Professor

Laboratory of Neurobiochemistry
Tel: +82-31-299-6134
Fax: +82-31-299-6139
e-mail: jyahn@med.skku.ac.kr

Education:

Sep.1997 - Feb.2000: Ph.D., Department of Biology, Hallym University, Korea
Sep.1995 - Aug.1997: M.S., Department of Genetic Engineering, Hallym University, Korea
Mar.1991 - Feb.1995: B.S., Department of Genetic Engineering, Hallym University, Korea

Experiences:

Mar.2005 - Present: Assistant Professor, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine
Oct.2002 - Jan.2005: Post doctoral Fellow, Department of Pathology and Laboratory, school of Medicine, Emory University Atlanta, GA, U.S.A.
Sep.2001 - Aug.2002: Research Professor, Institute of Medical Science Hallym University Chuncheon, Korea
Sep.2000 - Aug.2001: Research Professor, Research institute for gastroenterology, Dankook Univ. College of Medicine, Cheonan, Korea

Research Interest:

-Neuronal differentiation, plasticity, and cell survival in NGF mediated signal transduction
-Nuclear Akt regulation and biological functions

-Nuclear Ptdlns(3,4,5)P3 Signaling

We currently direct our much attention 1) to understand NGF signaling cascades in neuronal differentiation, plasticity and cell survival, 2) to address the molecular regulation and biological functions of nuclear Akt, and 3) to elucidate nuclear lipid signaling and search for its potential target. Nerve Growth Factor (NGF) regulates the development of several classes of neurons and mediates their maintenance and survival. However, the mechanisms through which NGF signals are propagated from plasma membrane to the nucleus, and their functions in regulating neuronal development, differentiation and survival remain obscure. NGF promotes neuronal survival by provoking a variety of signaling cascades including the PI 3-kinase(PI3K) /Akt pathway, which plays an essential role in this process. PI3K/Akt signaling block cell death by impinging on the cytoplasmic/nuclear apoptotic machinery and by mediating the expression of genes involved in cell death and survival. While much has been learned about the PI3K/Akt anti-apoptotic signal in the cytoplasm, little is known about the nuclear counterpart. The nuclei from NGF-treated PC12 cells resist apoptotic degradation elicited by active cell-free apoptotsome. Recently, we showed that B23 act as a nuclear receptor for PI(3,4,5)P3 and nuclear Akt contribute to suppressing apoptosis through interaction with Ebp1. However the molecular mechanism underlying this nuclear event remains elusive. Elucidation of their mechanisms of action in the cell death machinery in neurons not only leads to a better understanding of nervous system development but also promises to provide multiple points of therapeutic intervention for neurodegenerative diseases.

Publications:

  1. Xuan Nguyen TL, Choi JW, Lee SB, Ye K, Woo SD, Lee KH, Ahn JY.
    Akt phosphorylation is essential for nuclear translocation and retention in NGF-stimulated PC12 cells. Biochem Biophys Res Commun. 349: 789-798 (2006/10)
  2. Zhixue Liu, Jee-Yin Ahn, Xia Liu, and Keqiang Ye
    Ebp1 isoforms distinctively regulate cell survival and differentiation. Proc Natl Acad Sci. 103(29):10917-22 (2006/07)
  3. Jee-Yin Ahn, Xia Liu, Zhixue Liu, Lorena Pereira, Dongmei Cheng, Junmin Peng Paul A. Wade, Anne W. Hamburger and Keqiang Ye
    Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase EMBO Journal 25(10): 2083-2095 (2006/05)
  4. Jee-Yin Ahn, Xia Liu, Dongmei Cheng, Junmin Peng, Pui-Kwang Chan, Paul A. Wade and Keqiang Ye
    Nucleophosmin/B23, a Nuclear PI (3,4,5)P3 Receptor, Mediates the Antiapoptotic Actions of NGF by Inhibiting CAD Molecular Cell 18:435-445 ( 2005/05/13)
  5. Jee-Yin Ahn and Keqiang Ye
    PIKE GTPase signaling and function Int. J. Biol. Sci 1:44-50 (2005/4) Review paper
  6. Lee BR, Kim DW, Hong JW, Eum WS, Choi HS, Choi SH, Kim SY, An JJ, Ahn JY, Kwon OS, Kang TC, Won MH, Cho SW, Lee KS, Park J, Choi SY
    Brain Succinic Semialdehyde Dehydrogenase : Reactions of Sulfhydryl Residues Connected with Catalytic Activity European Journal of Biochemistry 271(23-24):4903-8 (2004/12)
  7. Dae Won Kim, Chang Sik Yoon, Won Sik Eum, Byung Ryong Lee, Jae Jin An, Sun Hwa Lee, Seung Ree Lee, Jee-Yin Ahn, Oh-Shin Kwon, Tae-Cheon Kang, Moo Ho Won, Sung-Woo Cho, Kil Soo Lee, Jinseu Park and Soo Young Choi
    Site-directed Mutatgenesis of Human Brain GABA Transaminase: Lysine-357 involved in cofactor binding at the active site Molecules and Cells 18(3):314-9 (2004/12)
  8. Jee-Yin Ahn, .Rong Rong Xuesong Liu, and Keqiang Ye
    PIKE/Nuclear PI 3-Kinase Signaling Mediates the antiapoptotic actions of NGF in the Nucleus The EMBO Journal 23(20); 3995-4006 (2004/10)
  9. Jee-Yin Ahn, Yuanxin Hu,Todd G. Kroll, Paulette Allard, and Keqiang Ye
    PIKE-A is amplified in human cancers and prevents apoptosis by up-regulating Akt Proceedings of National Academy of Sciences (PNAS) 101(18): 6993-6998(2004/5/4)
  10. Jee-Yin Ahn, .Rong Rong Todd G. Kroll, Van Meir Erwin, Solomon H Snyder and Keqiang Ye
    PIKE (Phosphatidylinositol 3-Kinase-enhancer)-A GTPase Stimulates Akt Activity and Mediates Cellular Invasion. Journal of Biological Chemistry. 16; 279(16): 16441-16451(2004/04)
  11. Rong Rong, Jee-Yin Ahn, Pong Chen, Pann Ghill Suh, and Keqiang Ye
    Phospholipase Activity of Phospholipase C-g1 is Required for Nerve growth factor -regulated MAP Kinase Signaling Cascade in PC12 Cells. Journal of Biological Chemistry. 26; 278(52): 52497-52503 ( 2003 /12)
  12. Rong Rong, Jee-Yin Ahn, Honglian Huang, Eiichiro Nagata, Daniel Kalman, Judith A Kapp, Jiancheng Tu, Pawl F Worley, Solomon H Snyder and Keqiang Ye
    PI 3 kinase enhancer-Homer complex couples mGluRl to PI 3 kinase, preventing neuronal apoptosis Nature Neuroscience 6(11), 1-9 (2003/11)
  13. J. H. Bahn, S. H. Jang, B. R. Lee, J. Y. Ahn, H.M. Joo, T-C Kang, M. H. Won, H. Y. Kwon, J. H. Kang, O-S Kwon, H. B. Kim, S-W. Cho, K. S. Lee, J. Park, and S. Y. Choi
    Production of Monoclonal Antibodies and Immunohistochemical Studies of Brain Myo-Inositol Monophosphate Phosphatase Molecules and Cells 13, 21-27 (2002/2)
  14. Eun-Young Lee, Hye-Young Yoon, Jee-Yin Ahn, Soo Young Choi, and Sung-Woo Cho
    Identification of GTP Binding Site of Human Glutamate Dehydrogenase by Cassette Mutagenesis and Photoaffinity Labeling Journal of Biological Chemistry 276(51), 47930-47936 (2001/12)
  15. Cho Sung-Woo, Yoon Hye-Young, Ahn Jee-Yin, Lee Eun-Young, Lee Jongwon
    Cassete mutagenesis of lysine 130 of human glutamate dehydrogenase. An essential residue in catalysis European Journal of Biochemistry 268(11), 3205-3213(2001/6)
  16. Im hwan Roe, Seung Woo Nam, Moon Sook Lee, Mie Rha Yang, Jung Taik Kim, Kyung Ae Yu, Ji Hyun Shin, Jong Hwa Lee and Jee-Yin Ahn
    Mosaicism in cagA Gene from Helicobacter pylori Isolates from Gastric Cancer Patients and Its Role in the Carcinogenesis Korean Journal of Gastroenterology 37, 7-13 (2001/1)
  17. Sung-Woo Cho, Jee-Yin Ahn, Jae Hoon Bahn, Seong Gyu Jeon , Jinseu Park, Kil Soo Lee, and Soo Young Choi
    Production and Charaterization of Monoclonal Antibodies to Glutamate Dehydrogenase from Thermophile Sulfolobus solfataricus Journal of Microbiology and Biotechnology 10, 587-594(2000/11)
  18. Jee-Yin Ahn, Kil Soo Lee, Soo Young Choi, and Sung-Woo Cho
    Regulatory Properties of Glutamate Dehydrogenase from Sulfolobus solfataricus. Molecules and Cells 10, 25-31. (2000/2)
  19. Jee-Yin Ahn, Soo Young Choi, and Sung-Woo Cho
    Identification of Lysine Residue Involved in Inactivation of Brain Glutamate Dehydrogenase Isoproteins by o-Phthalaldehyde Biochimie 81, 1-7. (1999/12)
  20. Jee-Yin Ahn, Eun Hee Cho, Kil Soo Lee, Soo Young Choi, and Sung-Woo Cho
    Chemical Modification of Brain Glutamate Dehydrogenase Isoproteins with Phenylglyoxal. Journal of Biochemistry and Molecular Biology 32, 515-520. (1999/9)
  21. Soo Young Choi, Joung Woo Hong, Min-Sun Song, Seong Gyu Jeon, Jae Hoon Bahn, Byung Ryong Lee, Jee-Yin Ahn, and Sung-Woo Cho
    Different Antigenic Reactivities of Bovine Brain Glutamate Dehydrogenase Isoproteins. Journal of Neurochemistry 72, 2162-2169. (1999/5)
  22. Sung-Woo Cho, Hye Young Yoon, Jee-Yin Ahn, Soo Young Choi, and Tae Ue Kim
    Identification of an NAD+ Binding Site of Brain Glutamate Dehydrogenase Isoproteins by Photoaffinity Labeling. Journal of Biological Chemistry 273, 31125-31130. (1998/11)
  23. Soo Young Choi, Jae Hoon Bahn, Seong Gyu Jeon, Young Mee Chung, Jung Woo Hong, Jee-Yin Ahn, Eunjoo Hwang Lee, Sung-Woo Cho, Jinkyu Park, and Nam-In Baek
    Stimulatory Effects of Ginsenosides on Bovine Brain Glutamate Decarboxylase. Journal of Biochemistry and Molecular Biology 31, 233-239. (1998/5)
  24. Byung Kwon Yoo, Joung Woo Hong, Jae Wook Suk, Jee-Yin Ahn, Sung-Woo Cho, and Soo Young Choi
    Inhibitory Actions of The AntidePressant/Antipanic Drug Phenelzine on Brain GABA Transaminase. Archives of Pharmacal Research 19, 480-485. (1996/12)
  25. Sung-Woo Cho, Jee-Yin Ahn, Jongweon Lee, and Soo Young Choi
    Identification of a Peptide of the Guanosine Triphosphate Binding Site within Brain Glutamate Dehydrogenase Isoproteins Using 8-Azidoguanosine Triphosphate. Biochemistry 35, 13907-13913. (1996/11)